GLIIFCA roundtable lunch workshops • •
1. Cell Sorting - Maximizing User (and Operator) Satisfaction
Dagna Shearer, Manager, UWCCC Flow Cytometry Laboratory
We will discuss methods that the UWCCC Flow Lab has implemented to make the sorting experience as efficient and painless as possible for both users and operators. Topics will include pre-sort consults, tech notes and protocols available to users, sort request forms, communication during the sort and post-sort surveys to measure user satisfaction. Examples of tech notes, protocols, sort request forms and surveys will be made available.
2. Considerations in Building and Analyzing Multicolor Flow Panels
Kelly Lundsten, Business Segment Manager, Advanced Cytometry, Biolegend
Constructing panels of more than 12 fluorescent parameters has a unique set of challenges than assays with less complexity. Building a balanced panel depends heavily on the context of the application and the path of analysis that’ll be used. We’ll also discuss practical concerns and experiences doing phospho-specific and transcription factor staining in a multicolor context in particular.
3. Microvesicle Detection, Isolation and Applications
Nancy Fisher, Flow Cytometry Core Director, University of North Carolina
Advances in flow cytometry instrumentation have allowed us to detect and measure small cell-derived particles. While technical challenges remain, a whole new biology is opening up toward understanding cancer, thrombosis, infectious diseases and basic cell biology. At the round table we will discuss basic approaches and challenges to MV detection for greatest confidence, current approaches for isolation and new biological insights.
4. Single Cell Sorting for Genomic Analysis
Rachael Sheridan, Director Flow Core Facility, Van Andel Institute, Grand Rapids, MI
As single cell genomic analysis capabilities increase, researchers are going to require more single cell sorting. We’ll discuss validation of sorter performance for single drop sorts into plates compatible with downstream genomic analysis to maximize success and minimize sample handling. There will be ample opportunity to share experiences with these sorts and the collaboration required with another core that may or may not be within the same institution.
5. SRL Best Practices
Monica DeLay, Lab Manager of Operations,Research Flow Cytometry Core, Cincinnati Children’s Hospital,Cincinnati, OH
In November 2016 an ISAC Shared Resource Laboratory (SRL) Task Force published a document outlining a set of “best practices” for SRLs or Core Facilities to use as a general guide for achieving and maintaining standards of excellence in the services they provide. These best practices highlight several important areas that impact the efficiency, quality and reproducibility of services provided by these facilities. The purpose of this roundtable is to generate discussion and feedback on how to best implement these “best practices” across a wide variety Shared Resource Labs.
6. SCYM Exam
Jessica Back, Associate Director, Microscopy,Imaging, and Cytometry Resources Core, Karmanos Cancer Institute, Wayne State University, MI
ISAC, the International Clinical Cytometry Society (ICCS), and the American Society for Clinical Pathology Board of Certification (ASCP BOC) recently introduced the SCYM, a new Specialist Certification in Cytometry launching in fall of 2017, which will replace the International Cytometry Certification Exam (ICCE) and the ASCP BOC’s Qualification in Cytometry (QCYM) recognition program. The process for transitioning current ICCE Certified Cytometrist (CCy) certificate holders to the new SCYM certification, credential maintenance, eligibility requirements for new applicants, as well as general information and frequently asked questions about the SCYM will be addressed.
7. What does it take to Monitor Response to Therapy?
Michael Loken, President, Laboratory Director, Hematologics, Inc., Seattle, WA
Based on a detailed understanding of normal gene product expression it is possible to detect 0.02% aberrant myeloblasts in regenerating bone marrow specimens, specifically, even without access to a diagnostic specimen. However, this approach requires system stability and QC not usually found in most laboratories.
8. How to get Computational Biology to Benchtop Scientists
Josef Spidlen, Director of Bioinformatics, FlowJo, LLC, Ashland, OR
Many automated cytometry data analysis methods have been developed in the past decade and it’s been shown that those can work very well, and yet they are rarely used. Why is that? Lack of awareness? Do we need more training? Do the algorithms need to get better? What are the main issues and how can we address them?
9. Flow Virometry
John “Chip” Tilton, Assistant Professor, Center for Proteomics and Bioinformatics, School of Medicine, Case Western Reserve University, Cleveland OH
Flow cytometric analysis of submicron particles, including microvesicles, exosomes, and viruses, faces multiple technical challenges related to their small size and comparatively few numbers of antigen binding sites. This roundtable will discuss these technical challenges and their potential solutions and will attempt to provide perspective on the importance of understanding the heterogeneity and biological function of small extracellular vesicles and viruses
10. Integration of Flow Cytometry Data into other Omics Platforms
Nima Aghaeepour,Baxter Laboratory in Stem Cell Biology, Stanfory University, CA
High-dimensional flow cytometry is often measured together with other data modalities (e.g. from the transcriptome, microbiome, and metabolome) of the same patient cohort. We will discuss several possible strategies for combining these modalities to produce a holistic understanding of the entire dataset.
11. Amnis Imaging Flow Cytometry: Emerging Applications and Analysis Tools
Robert Thacker, Amnis Corporation, MilliporeSigma
Come join in on a discussion that will cover some of the latest applications, get your questions answered about current applications, and learn about the latest features and masks in IDEAS designed to enhance the depth of your data analysis.
12. Building Panels for Mass Cytometry: Tools and Tips,
Clare Rogers, Marketing Manager, Mass Cytometry, Fluidigm Corporation
This roundtable will address the key requirements to successfully build and optimize mass cytometry panels for moderate- (15–25) as well as high-parameter (30-plus) analysis. The session will include an overview of preconfigured panel kits currently available as well as an introduction to the tools and resources available to support all aspects of mass cytometry from experimental design to data analysis.
Page updated on 2017-08-25 09:03:03 -0400